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jagged 1 protein  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc jagged 1 protein
    Jagged 1 Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jagged+1/Jagged1+XP+Rabbit+mAb/us12590307-1297-0-9
    Average 95 stars, based on 135 article reviews
    jagged 1 protein - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Membrane:

    Article Title: Study of dietary‑induced progression of psoriasis‑like mice based on gut macrophage polarization
    Article Snippet: Total protein content in the tissue lysates were determined by a bicinchoninic acid assay kit (cat. no. AR0146; Wuhan Boster Biological Technology, Ltd.) and mixed with loading buffer when subjected to SDS-PAGE on Bolt 10% Bis-Tris Plus Gels (Invitrogen; Thermo Fisher Scientific, Inc.). .. Proteins were transferred onto a PVDF membrane (MilliporeSigma), and then incubated with primary antibodies specific for NF-κB p65 (cat. no. 6956), phospho-NF-κB p65 (cat. no. 3033), Toll-like receptor-2 (cat. no. 2229S), IKBα (cat. no. 9242), Jagged-1 (cat. no. 70109s), Notch-1 (cat. no. 3608s; all CST; 1:1,000), Hes-5 (cat. no. ab25374; Abcam; 1:1,000) and anti-β-actin (cat. no. BM0627; Wuhan Boster Biological Technology, Ltd.; 1:2,000) overnight at 4 ̊C and then followed by incubation with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit IgG (cat. nos. .. 7076 and 7074; CST; 1:2,000) at room temperature for 2 h. The blots were visualized by Beyo ECL Star (cat. no. P0018AS; Beyotime Institute of Biotechnology) with Bio-Rad Gel Doc XR+ and quantified with Image Lab software (Bio-Rad Laboratories, Inc.).

    Article Title: Study of dietary‑induced progression of psoriasis‑like mice based on gut macrophage polarization.
    Article Snippet: Total protein content in the tissue lysates were determined by a bicinchoninic acid assay kit (cat. no. AR0146; Wuhan Boster Biological Technology, Ltd.) and mixed with loading buffer when subjected to SDS‐PAGE on Bolt 10% Bis‐Tris Plus Gels (Invitrogen; Thermo Fisher Scientific, Inc.). .. Proteins were transferred onto a PVDF membrane (MilliporeSigma), and then incubated with primary antibodies specific for NF‐κB p65 (cat. no. 6956), phospho‐NF‐κB p65 (cat. no. 3033), Toll‐like receptor‐2 (cat. no. 2229S), IKBα (cat. no. 9242), Jagged‐1 (cat. no. 70109s), Notch‐1 (cat. no. 3608s; all CST; 1:1,000), Hes‐5 (cat. no. ab25374; Abcam; 1:1,000) and anti‐β‐actin (cat. no. BM0627; Wuhan Boster Biological Technology, Ltd.; 1:2,000) overnight at 4 ̊C and then followed by incubation with horseradish peroxidase‐conjugated secondary anti‐mouse or anti‐rabbit IgG (cat. nos. .. 7076 and 7074; CST; 1:2,000) at room temperature for 2 h. The blots were visualized by Beyo ECL Star (cat. no. P0018AS; Beyotime Institute of Biotechnology) with Bio‐Rad Gel Doc XR+ and quantified with Image Lab software (Bio‐Rad Laboratories, Inc.).

    Incubation:

    Article Title: Study of dietary‑induced progression of psoriasis‑like mice based on gut macrophage polarization
    Article Snippet: Total protein content in the tissue lysates were determined by a bicinchoninic acid assay kit (cat. no. AR0146; Wuhan Boster Biological Technology, Ltd.) and mixed with loading buffer when subjected to SDS-PAGE on Bolt 10% Bis-Tris Plus Gels (Invitrogen; Thermo Fisher Scientific, Inc.). .. Proteins were transferred onto a PVDF membrane (MilliporeSigma), and then incubated with primary antibodies specific for NF-κB p65 (cat. no. 6956), phospho-NF-κB p65 (cat. no. 3033), Toll-like receptor-2 (cat. no. 2229S), IKBα (cat. no. 9242), Jagged-1 (cat. no. 70109s), Notch-1 (cat. no. 3608s; all CST; 1:1,000), Hes-5 (cat. no. ab25374; Abcam; 1:1,000) and anti-β-actin (cat. no. BM0627; Wuhan Boster Biological Technology, Ltd.; 1:2,000) overnight at 4 ̊C and then followed by incubation with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit IgG (cat. nos. .. 7076 and 7074; CST; 1:2,000) at room temperature for 2 h. The blots were visualized by Beyo ECL Star (cat. no. P0018AS; Beyotime Institute of Biotechnology) with Bio-Rad Gel Doc XR+ and quantified with Image Lab software (Bio-Rad Laboratories, Inc.).

    Article Title: Study of dietary‑induced progression of psoriasis‑like mice based on gut macrophage polarization.
    Article Snippet: Total protein content in the tissue lysates were determined by a bicinchoninic acid assay kit (cat. no. AR0146; Wuhan Boster Biological Technology, Ltd.) and mixed with loading buffer when subjected to SDS‐PAGE on Bolt 10% Bis‐Tris Plus Gels (Invitrogen; Thermo Fisher Scientific, Inc.). .. Proteins were transferred onto a PVDF membrane (MilliporeSigma), and then incubated with primary antibodies specific for NF‐κB p65 (cat. no. 6956), phospho‐NF‐κB p65 (cat. no. 3033), Toll‐like receptor‐2 (cat. no. 2229S), IKBα (cat. no. 9242), Jagged‐1 (cat. no. 70109s), Notch‐1 (cat. no. 3608s; all CST; 1:1,000), Hes‐5 (cat. no. ab25374; Abcam; 1:1,000) and anti‐β‐actin (cat. no. BM0627; Wuhan Boster Biological Technology, Ltd.; 1:2,000) overnight at 4 ̊C and then followed by incubation with horseradish peroxidase‐conjugated secondary anti‐mouse or anti‐rabbit IgG (cat. nos. .. 7076 and 7074; CST; 1:2,000) at room temperature for 2 h. The blots were visualized by Beyo ECL Star (cat. no. P0018AS; Beyotime Institute of Biotechnology) with Bio‐Rad Gel Doc XR+ and quantified with Image Lab software (Bio‐Rad Laboratories, Inc.).

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    other:

    Article Title: Proprotein Convertase Furin Regulates Melanogenesis via the Notch Signaling Pathway
    Article Snippet: Antibodies againstMITF (12590S, 1:4000), Rab27A (69295S, 1:1000), Myo5a (3402S, 1:1000), FSCN1 (99978S, 1:1000), Notch1 (4380T, 1:1000), Notch-2 (5732T, 1:1000), Notch3 (5276T, 1:1000), Jagged-1 (2620T, 1:1000), Jagged2 (2210T, 1:1000), delta-like ligand 1 (DLL-1) (2588T, 1:1000), delta-like ligand (DLL-4) (2589T, 1:1000), hairy and enhancer of split-1 (Hes-1) (11988S, 1:1000), Cleaved Notch-1 (N1ICD) (4147S, 1:1000), and β-actin (4970S, 1:2000) were obtained from Cell Signaling Technology (Danvers, MA, USA).

    Article Title: eNOS plays essential roles in the developing heart and aorta linked to disruption of Notch signalling
    Article Snippet: Sections were cut from paraffin wax-embedded embryos at 8 μm using a rotary microtome (Leica).

    Article Title: Tspan5 promotes the EMT process to regulate the syncytialization of trophoblast cells by activating Notch signalling
    Article Snippet: Placental trophoblastic cells play important roles in placental development and fetal health.. However, the mechanism of trophoblastic cell fusion is still not entirely clear.. The level of Tspan5 in the embryo culture medium was detected using enzyme-linked immunosorbent assay (ELISA).

    Lysis:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    SDS Page:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    Saline:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.



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    Image Search Results


    NOTCH pathway modulation affects HG‐induced PANoptosis. hRMECs were treated with a NOTCH agonist (Jagged1 peptide) or inhibitor (DAPT) under HG conditions. (A) Cell viability measured by CCK‐8 assay. (B) Caspase‐3/7 activity measured by colorimetric assay. (C) IL‐1 β and IL‐18 levels were quantified by ELISA. (D) Apoptosis rate measured by flow cytometry with quantification. (E) Quantification of Western blot analysis for PANoptosis‐related proteins, including cleaved Caspase‐3, GSDMD‐N, RIPK1, Bax, RIPK3, p‐MLKL, and Bcl‐2. (F) Co‐IP analysis of PANoptosome components (ASC, Caspase‐1, NLRP3, and RIPK3). (G) qPCR analysis of Bax and Bcl-2 mRNA levels. (H, I) IF staining of p‐MLKL (green) with DAPI (blue) and quantification. Scale bar = 150 μ m. Data are presented as mean ± SD ( n = 3). Statistical analysis was performed using one‐way ANOVA followed by Tukey′s post hoc test. ∗ p < 0.01, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns = not significant.

    Journal: Journal of Diabetes Research

    Article Title: CD36 Regulates PANoptosis in Diabetic Retinopathy via the NOTCH/MAML Pathway

    doi: 10.1155/jdr/9324498

    Figure Lengend Snippet: NOTCH pathway modulation affects HG‐induced PANoptosis. hRMECs were treated with a NOTCH agonist (Jagged1 peptide) or inhibitor (DAPT) under HG conditions. (A) Cell viability measured by CCK‐8 assay. (B) Caspase‐3/7 activity measured by colorimetric assay. (C) IL‐1 β and IL‐18 levels were quantified by ELISA. (D) Apoptosis rate measured by flow cytometry with quantification. (E) Quantification of Western blot analysis for PANoptosis‐related proteins, including cleaved Caspase‐3, GSDMD‐N, RIPK1, Bax, RIPK3, p‐MLKL, and Bcl‐2. (F) Co‐IP analysis of PANoptosome components (ASC, Caspase‐1, NLRP3, and RIPK3). (G) qPCR analysis of Bax and Bcl-2 mRNA levels. (H, I) IF staining of p‐MLKL (green) with DAPI (blue) and quantification. Scale bar = 150 μ m. Data are presented as mean ± SD ( n = 3). Statistical analysis was performed using one‐way ANOVA followed by Tukey′s post hoc test. ∗ p < 0.01, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns = not significant.

    Article Snippet: To modulate NOTCH signaling under HG conditions, cells were cultured in 25.0 mmol/L D‐glucose for 72 h. During the final 24 h, the HG group was supplemented with either 5 μ g/mL Jagged1 peptide (MedChemExpress, United States) to activate the pathway or 10 μ M DAPT (MedChemExpress, United States) to inhibit it.

    Techniques: CCK-8 Assay, Activity Assay, Colorimetric Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Co-Immunoprecipitation Assay, Staining